Bacterial Selection and Plasmid Maintenance: Used as an antibiotic to select and maintain tet-resistance plasmids in Escherichia coli and other bacteria.

Inducible Gene Expression (Tet-On/Tet-Off): Regulates transcription in tetracycline-responsive systems in mammalian and microbial cells.

Antimicrobial Susceptibility Testing: Utilized in MIC determination and disk diffusion assays against organisms such as Staphylococcus aureus and Escherichia coli.

Mycoplasma Control in Cell Culture: Applied in treatment protocols to eliminate Mycoplasma contamination.

Protein Synthesis Inhibition Studies: Employed to inhibit bacterial ribosomes and mitochondrial translation for mechanistic studies.

Selective Media Supplementation: Added to agar or broth to create tetracycline-selective media for isolating or maintaining resistant strains.

Contamination Suppression in Mixed Cultures: Used to suppress unwanted bacterial growth in yeast or eukaryotic cell workflows involving Escherichia coli.

Stock Solution Preparation for Media: Prepared as sterile-filtered solutions for routine supplementation of culture media.