Polymerase Chain Reaction (PCR): Used as a 200mM Tris, pH 7.0 stock to maintain stable pH in PCR and RT-PCR master mixes.

Gel Electrophoresis: Serves as a Tris source to prepare Tris-based running buffers (e.g., TAE/TBE, Tris–Glycine) for nucleic acid and protein separation.

Nucleic Acid Handling: Provides a neutral 200mM Tris buffer for DNA/RNA resuspension, dilution, and storage to minimize acid hydrolysis.

Protein Purification: Used to formulate Tris-based equilibration, wash, and elution buffers for ion-exchange and affinity chromatography at controlled pH.

Enzyme Assays: Supplies a defined 200mM Tris, pH 7.0 environment for enzymatic reactions and kinetic measurements near neutral pH.

Western Blotting: Used to prepare Tris-buffered saline (TBS/TBST) and Tris–Glycine buffers for transfer and membrane washes.

Cell Lysis and Sample Prep: Included in lysis and extraction buffers for recombinant protein prep from Escherichia coli and other bacteria.

Buffer Preparation and pH Adjustment: Acts as a sterile 200mM Tris stock to titrate and standardize Tris-based buffers at pH 7.0.