Antibiotic Selection in Bacteria: Selects tetracycline-resistant transformants and maintains selection in E. coli, Agrobacterium tumefaciens, and Bacillus subtilis.

Plasmid Maintenance in Culture: Preserves plasmids bearing tetracycline resistance markers during growth in liquid and solid media.

Agrobacterium-Mediated Plant Transformation: Maintains binary vectors and selects engineered Agrobacterium strains used for plant gene delivery.

Tet-Regulated Gene Expression: Serves as the effector molecule in Tet-Off/Tet-On systems for controlled gene expression in bacterial and mammalian cells.

Selective Pressure in Mixed Microbial Cultures: Suppresses tetracycline-sensitive background flora to enrich desired resistant strains.