Polymerase Chain Reaction (PCR/qPCR): Provides a stable pH 8.5 environment for DNA polymerase performance and template dilution.

Reverse Transcription (RT): Maintains optimal pH during RNA dilution and cDNA synthesis setup.

Nucleic Acid Resuspension and Storage: Used to resuspend and store DNA or RNA, preserving integrity and stability.

Spin-Column and Gel Extraction Elution: Elutes DNA from silica membranes or gels with gentle, low-ionic-strength conditions.

Restriction Digests and Ligation Setup: Serves as a dilution buffer for enzymes and DNA to maintain consistent reaction conditions.

Library Preparation for NGS: Dilutes adapters, primers, and DNA fragments to ensure accurate molarity and pH control.

Enzyme Assays: Provides a defined pH for biochemical assays requiring Tris-based buffering.

Sample Normalization and Standards: Used to prepare calibration curves and control dilutions for quantitative analyses.

Buffer Component for Tris-Based Systems: Acts as a base buffer for preparing TAE/TBE variants or Tris-adjusted solutions.

Bacterial Lysate Preparation: Included in lysis and wash buffers for processing samples from Escherichia coli and other microbes.