Polymerase Chain Reaction (PCR): Used to formulate and adjust PCR buffers, maintaining pH near neutrality for polymerase stability.

Gel Electrophoresis: Used to prepare Tris-based running and gel buffers (e.g., Tris–glycine, Tris–acetate) for consistent pH during nucleic acid and protein separation.

Protein Purification: Employed in ion-exchange and affinity chromatography buffers to stabilize pH during binding, wash, and elution steps.

Enzyme Assays: Provides a stable pH 7.5 environment for enzymatic reactions and kinetic measurements.

Western Blotting: Used in transfer buffers and Tris-buffered saline (TBS/TBST) for protein transfer and membrane washes.

Nucleic Acid Workflows: Used to prepare TE and other Tris-based buffers for DNA/RNA extraction, purification, and short-term storage.

Restriction Digestion: Provides Tris-based buffering in digestion mixes to maintain enzyme activity and DNA integrity.

Ligation Reactions: Supplies the Tris-HCl component at pH 7.5 commonly used in DNA ligase buffers for efficient ligation.

Cell Lysis and Protein Extraction: Included in lysis and extraction buffers to preserve protein stability at near-neutral pH.

General Buffer Preparation: Serves as a convenient 2M stock to titrate and prepare working Tris buffers at pH ~7.5 for diverse biochemical assays.