Selection and Maintenance of Ampicillin-Resistant Clones: Selects and maintains AmpR plasmid-bearing Escherichia coli after transformation.

Plasmid Cloning Workflows: Used for recovery plating and clone isolation following ligation, Gibson, or Golden Gate assembly.

Colony Isolation and Purification: Streaking to single colonies to obtain clonal AmpR strains for downstream work.

Colony PCR and Screening: Picking AmpR colonies for PCR, restriction analysis, or Sanger sequencing.

CFU Enumeration: Quantifying viable AmpR cells to assess transformation efficiency or library diversity.

Expression Screening: Evaluating expression constructs in AmpR Escherichia coli strains under selective pressure.

Maintenance of Reference Strains: Routine passaging of AmpR lab strains to prevent plasmid loss.

Contamination Control in Mixed Samples: Suppresses ampicillin-sensitive microbes, enriching target AmpR populations.

Phage and Host Workflows with Selection: Preserves AmpR plasmids in host lawns during bacteriophage assays using suitable AmpR hosts such as Escherichia coli.

Pre-culture and Stock Preparation: Starting clonal cultures from selected colonies for glycerol stocks or cryostorage.