Polymerase Chain Reaction (PCR): Component of PCR/qPCR buffers to maintain alkaline pH for polymerase activity.

Gel Electrophoresis: Resolving gel buffer for SDS-PAGE (Tris-HCl pH 8.8) and preparation of Tris–glycine electrophoresis buffers.

Protein Purification: Buffering agent for anion-exchange chromatography and protein elution at alkaline pH.

Enzyme Assays: Maintains stable pH for enzymatic reactions and protein stability studies.

Western Blotting: Preparation of Tris–glycine transfer buffers and Tris-buffered saline (TBS) after dilution/adjustment.

Nucleic Acid Buffers: Preparation of Tris-based DNA/RNA resuspension and storage buffers (e.g., TE) by dilution to working concentrations.