1.5 M Tris-HCl, pH 10.0 is a concentrated Tris buffer solution for preparing alkaline working buffers by dilution. It provides pH control in molecular biology and biochemistry workflows that specify Tris at pH 10.0, including sample preparation, protein extraction, enzyme assays requiring alkaline conditions, and electrophoresis or transfer buffers formulated with Tris components.

The solution can be diluted with high-purity water to the desired working concentration. Because Tris pH is temperature dependent, equilibrate to the intended use temperature before final pH verification. Store tightly capped to limit CO₂ absorption and pH drift; avoid contamination by using clean, appropriate labware.