Aerobic Bacterial Cultivation and Enrichment: Broad-growth broth for routine culture of organisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Enterococcus faecalis, Salmonella enterica, and Listeria monocytogenes.

Sterility Testing (USP <71>): Ready-to-use sterile broth for detecting microbial contamination in pharmaceutical and medical device samples.

Microbial Limits/Bioburden Testing: Enrichment medium for assessing total aerobic counts in raw materials, intermediates, and finished products.

Environmental Monitoring and Swab Rinse: Surface and equipment swab recovery; 0.10% Tween-80 helps release and disperse adherent microbes.

Membrane Filtration Rinse and Sample Dilution: Rinse/diluent for filter-based testing of water and cleanroom samples to enhance organism recovery.

Disinfectant Neutralization and Recovery: Polysorbate 80 aids neutralization of residual sanitizers and improves recovery of stressed organisms during validation.

Disinfectant Efficacy/Validation Studies: Used in time-kill and surface challenge tests (e.g., ASTM/AOAC methods) as a recovery/enrichment medium.

Bacteriostasis/Fungistasis (B/F) Testing: Employed to verify that test articles do not inhibit microbial growth in sterility assays.

Food and Beverage Pathogen Enrichment: Pre-enrichment of samples prior to selective plating for organisms including Salmonella enterica and Listeria monocytogenes.

Cosmetic and Personal Care Testing: Enumeration and preservative-neutralization support in product testing where polysorbate 80 improves recovery.