General Bacterial Cultivation: Rich, non-selective medium for robust growth of aerobes and facultative anaerobes such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis.

USP Sterility Testing (Soybean-Casein Digest Medium): Employed per USP <71> for sterility tests of pharmaceuticals, biologics, and membrane filtration samples.

Bioburden and Microbial Limits Testing: Used as an enrichment and recovery broth for USP <61>/<62> microbial enumeration and specified microorganisms tests.

Environmental Monitoring and Swab Rinses: Recovery of low-level or stressed microbes from surfaces, equipment, and cleanroom EM swabs.

Inoculum Preparation: Routine propagation of cultures to generate standardized inocula for downstream assays (e.g., MIC setup, biochemical tests).

Growth Promotion Testing (GPT): Quality control of media performance using indicator strains including Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.

Product and Device Recovery Studies: Extraction broth for microbial recovery in disinfectant efficacy, preservative efficacy (USP <51>), and medical device validation.

Food, Beverage, and Water Testing: Non-selective pre-enrichment and cultivation of diverse heterotrophic bacteria, including stressed organisms.

Blood Culture and Clinical Microbiology Workflows: Base medium for cultivating clinical isolates and for blood culture systems when appropriately supplemented.

Maintenance and Short-Term Holding: Temporary culture maintenance and subculturing prior to long-term storage or analysis.