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T1530

Tryptic Soy Broth. 100mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

100mL

Item Count

1 Bottle(s)

Price per Item

$30.00

Fill Volume

100 mL

T1530

Tryptic Soy Broth. 100mL, Sterile.

Quantity

Minimum is set to 20 for this product*

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Description
Formulation
Common Applications
Storage

Tryptic Soy Broth is a nutrient-rich liquid medium for the cultivation of a broad range of aerobic and facultative anaerobic bacteria. It is commonly used for routine culture, microbial enumeration, and maintenance of organisms including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, and Bacillus subtilis.

The formulation combines enzymatic digest of casein and soybean meal as sources of peptides and amino acids, a carbohydrate source to support energy metabolism, sodium chloride to maintain osmotic balance, and a phosphate buffer to help stabilize pH during growth.

  • 1.70% Tryptone
  • 0.30% Soytone
  • 0.25% Glucose
  • 0.50% Sodium Chloride
  • 0.25% Potassium Phosphate Dibasic
  • Microbial Cultivation and Enrichment: General-purpose growth of aerobes and facultative anaerobes, including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, Enterococcus faecalis, and Bacillus subtilis.

  • Sterility Testing (USP/EP/JP): Detection of microbial contamination in pharmaceuticals, biologics, water, and process intermediates.

  • Bioburden and Environmental Monitoring: Enrichment of organisms recovered from swabs, surfaces, air, and water samples.

  • Food and Beverage Microbiology: Pre-enrichment and enrichment of pathogens and spoilage bacteria from foods, ingredients, and production environments.

  • Antimicrobial Susceptibility Testing: Broth-based growth controls and organism preparation for dilution methods.

  • Growth Promotion and QC Testing: Positive control medium for validating culture media performance and disinfectant efficacy.

  • Resuscitation of Stressed Cells: Recovery of sublethally injured bacteria following processing or sanitization.

  • Clinical Microbiology: Non-selective enrichment from clinical specimens and as a base medium for supplemented blood culture systems.

  • Starter Culture and Seed Inoculum Prep: Expansion of non-fastidious bacterial cultures for downstream applications.

  • Molecular Assay Pre-culture: Culture amplification prior to detection workflows such as PCR or sequencing.

  • Water and Utility Monitoring: Enrichment of bacteria from potable, process, and purified waters.

  • Short-term Culture Maintenance: Holding and routine subculture of laboratory strains and production isolates.

Storage Temperature
Room Temperature
Shelf Life
730 days
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