General Bacterial Cultivation: Broad-spectrum growth of nonfastidious organisms (e.g., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis).

Sterility Testing: Detection of microbial contamination in pharmaceuticals, reagents, and medical devices.

Environmental Monitoring/Bioburden: Recovery of microorganisms from surface swabs, air sampling media, and rinse samples.

Enrichment Prior to Plating: Pre-enrichment of low-level contaminants (e.g., Salmonella enterica, Enterococcus faecalis) before selective agar plating.

Quality Control of Cultures: Routine propagation and maintenance of reference strains (e.g., ATCC) for QC assays.

Antimicrobial Susceptibility/Challenge Studies: Preparation of inocula and growth controls in efficacy testing of disinfectants and antibiotics.

Membrane Filtration Recovery: Growth medium for recovering stressed or filter-captured microbes from water and process samples.

Food and Beverage Microbiology: Enrichment of indicator and spoilage organisms from foods, ingredients, and in-process samples.

Clinical/Diagnostic Microbiology: Nonselective broth for primary culture and subculture of clinical isolates.

Cryopreservation/Strain Banking Prep: Pre-growth of cultures prior to glycerol stock preparation or lyophilization.