Nucleic Acid Resuspension and Storage: Used to resuspend and store DNA or RNA at stable pH for long-term integrity.

DNA Elution (Spin/Gel Purification): Serves as an elution buffer for purified or gel-extracted DNA to maximize downstream enzymatic performance.

PCR/qPCR Template and Oligo Dilution: Maintains pH when diluting DNA templates, primers, and probes for amplification workflows.

Sanger/NGS Library Prep: Provides a low-salt, pH-stable medium for storing and diluting libraries, adapters, and barcodes.

Restriction/Ligation Workflows: Used to dilute DNA fragments and vectors without introducing inhibitory salts before enzyme reactions.

Protein Purification: Functions as a low-ionic-strength buffer for anion exchange equilibration, sample dilution, and gentle elution at pH 8.5.

Enzyme Assays: Supplies a defined pH environment for pH-sensitive enzymatic reactions and activity measurements.

Dialysis and Desalting: Employed as an exchange buffer to remove salts or small molecules while preserving biomolecule stability.

Plasmid DNA Handling: Used for resuspending and storing plasmid DNA isolated from Escherichia coli for reliable downstream use.

Electroporation-Compatible DNA Prep: Provides a low-conductivity buffer to prepare DNA for transformation while minimizing arcing risk.