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T1180

10mM Tris-HCl, pH 8.0. 2mL Tubes, 100-Pack, Sterile. DNase/RNase Tested.

Availability

Ships in 1 - 2 Days

Container Size

2mL

Item Count

100 Tube(s)

Price per Item

$359.00

Fill Volume

2 mL

T1180

10mM Tris-HCl, pH 8.0. 2mL Tubes, 100-Pack, Sterile. DNase/RNase Tested.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

Tris-HCl buffer at pH 8.0, DNase/RNase tested, is a commonly used buffering solution for molecular biology workflows. Each lot is screened for DNase and RNase contamination to help maintain nucleic acid integrity during handling.

Applications include preparation and storage of DNA and RNA, sample dilution, column equilibration, and buffering for enzymatic procedures such as restriction digestion, ligation, PCR, reverse transcription, and electrophoresis.

  • 10mM Tris-HCl
  • Nucleic Acid Resuspension and Storage: Used to dissolve and store DNA or RNA while maintaining stable pH and nuclease-free conditions.

  • DNA/RNA Dilution for PCR/qPCR: Serves as a low-salt diluent for templates, primers, and probes to preserve integrity and consistency in amplification workflows.

  • Column Elution Buffer: Employed for eluting purified nucleic acids from spin columns and magnetic beads after cleanup steps.

  • Restriction Digest and Ligation Setup Diluent: Provides a gentle, defined buffer for adjusting concentrations of DNA and enzymes in cloning workflows.

  • NGS and Library Prep: Used to dilute adapters, oligos, and libraries to working concentrations without introducing inhibitory salts.

  • Plasmid Prep and Transformation: Applied for resuspending plasmid DNA for transformation into Escherichia coli and other laboratory strains.

  • RNA Workflows and cDNA Synthesis: Supports RNA handling and primer dilution for reverse transcription while minimizing nuclease risk.

  • General Sample Preparation: Provides consistent pH control in low-ionic-strength conditions for biochemical and molecular biology procedures.

  • pH: 8.00 ± 0.04
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
1095 days
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