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T1173

10mM Tris-HCl Solution, pH 8.0. 1000mL, Sterile

Availability

Ships in 1 - 2 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$67.00

Fill Volume

1000 mL

T1173

10mM Tris-HCl Solution, pH 8.0. 1000mL, Sterile

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

Tris-HCl buffer is a laboratory solution used as a general-purpose buffering agent for molecular biology and biochemistry workflows.

It is used for preparation and dilution of DNA and RNA samples, resuspension of nucleic acids following purification, and as a base buffer in electrophoresis and enzyme reaction setups.

It provides a stable, mildly alkaline environment compatible with Tris-based buffer systems and routine handling of nucleic acids and proteins.

  • 10mM Tris-HCl
  • Nucleic Acid Resuspension and Storage: Maintains pH 8.0 to stabilize plasmid DNA, gDNA, and RNA during short- and long-term storage.

  • PCR/qPCR Template Dilution: Provides a low-salt, pH-stable medium for diluting DNA/RNA templates and standards.

  • Primer and Oligo Reconstitution: Used to rehydrate primers, probes, and oligonucleotides while preserving integrity.

  • DNA Elution from Silica Columns: Common elution buffer for purified DNA recovery at pH 8.0.

  • Restriction Enzyme and Ligation Diluent: Serves as a gentle buffering diluent to maintain pH in enzyme reaction setups.

  • Plasmid Prep and Sample Handling: Used for resuspension and neutral pH maintenance in workflows involving plasmid DNA.

  • Bacterial Transformation Workflows: Resuspends plasmid DNA for transformation into Escherichia coli and other laboratory strains.

  • Protein Purification and Dialysis Base Buffer: Serves as a starting buffer for column equilibration, sample prep, and dialysis at pH 8.0.

  • Spectrophotometry Baseline/Blank: Used as a reference blank for nucleic acid and protein absorbance measurements.

  • pH: 8.00 ± 0.10
Storage Temperature
Room Temperature
Shelf Life
1095 days
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