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T1128

10mM Tris, 1mM EDTA, 2.5M NaCl, 20% PEG 8000, 0.05% Tween-20, pH 8.0, 1000mL, D/R Tested, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$122.00

Fill Volume

1000 mL

T1128

10mM Tris, 1mM EDTA, 2.5M NaCl, 20% PEG 8000, 0.05% Tween-20, pH 8.0, 1000mL, D/R Tested, Sterile.

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Storage

Tris-EDTA–NaCl buffer with PEG 8000 and Tween-20, pH 8.0, D/R Tested. This formulation provides a high-salt, polyethylene glycol environment suitable for PEG/NaCl-mediated precipitation and concentration of bacteriophages and nucleic acids from aqueous solutions, and can be used as a binding or wash buffer in library construction and purification workflows.

D/R Tested indicates the lot is tested and found free of detectable DNase and RNase activity.

  • 10mM Tris
  • 1mM EDTA Disodium Dihydrate
  • 2.5M Sodium Chloride
  • 20% (w/v) PEG 8000
  • 0.05% (v/v) Tween-20
  • Bacteriophage Precipitation and Concentration: PEG 8000/NaCl-mediated precipitation of phage (e.g., M13/f1, λ, T7) from E. coli culture supernatants and lysates for downstream purification and titering.

  • Phage Display Workflows: Recovery and concentration of filamentous phage particles during phagemid library rescue and amplification from infected E. coli.

  • Size-Selective DNA Cleanup: PEG/NaCl-based precipitation of nucleic acids (PCR products, plasmid DNA, ssDNA) for cleanup and concentration.

  • Nucleic Acid Concentration from Dilute Samples: TE-stabilized precipitation to enrich DNA/RNA while inhibiting nuclease activity.

  • Removal of Enzymes and Proteins: PEG-induced precipitation to clear proteins from molecular biology reactions prior to DNA recovery.

  • Pre-clarification Before Ultracentrifugation: Aggregation/precipitation step to reduce sample volume and debris in phage prep workflows.

  • Reduced Nonspecific Adsorption: Tween-20 minimizes loss of particles and nucleic acids during handling, filtration, and storage.

Storage Temperature
Refrigerated
Shelf Life
1095 days
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