Polymerase Chain Reaction (PCR): Used in PCR reactions to maintain optimal pH conditions for enzyme activity.

Gel Electrophoresis: Provides Tris-based buffering for TAE/TBE and gel loading buffers to ensure consistent pH during DNA/RNA separation.

DNA/RNA Storage (TE Buffer): Forms the Tris component of TE buffer for resuspending and long-term storage of nucleic acids at pH 8.0.

Restriction Digests and Ligation: Maintains stable pH in cloning workflows, supporting restriction enzymes and DNA ligase activity.

Protein Purification: Used to make Tris buffers for ion-exchange and affinity chromatography, stabilizing protein structure during purification.

Enzyme Assays: Provides a consistent pH environment for accurate kinetic measurements and enzyme stability.

Western Blotting: Prepares Tris-Glycine(-SDS) running and transfer buffers, ensuring reliable protein separation and transfer.

TBS/TBST Preparation: Serves as the Tris base for Tris-buffered saline (with/without Tween 20) used in immunoassays and blot washing steps.

Cell Lysis Buffers: Constituent of Tris-based lysis buffers (e.g., RIPA) for protein extraction while maintaining pH 8.0.

Plasmid DNA Workflows: Used in neutralization and TE steps for plasmid preparation from Escherichia coli cultures.