Polymerase Chain Reaction (PCR): Used as a buffering component to maintain optimal pH conditions for polymerase activity.

Gel Electrophoresis: Employed in preparing Tris-based running and gel buffers for PAGE, providing consistent pH during protein and nucleic acid separation.

Protein Purification: Utilized in ion-exchange chromatography and general buffer preparation to maintain a stable pH environment during protein binding and elution.

Enzyme Assays: Provides a reliable alkaline buffer (pH 9.2) for enzymatic reactions, including alkaline phosphatase assays and other pH-sensitive activities.