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T1075

1M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$70.00

Fill Volume

1000 mL

T1075

1M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

1 M Tris-HCl, pH 7.5 Solution is a concentrated buffering reagent formulated to maintain a stable pH near neutrality in biological and biochemical workflows. At pH 7.5, Tris-HCl provides effective buffering capacity for applications requiring controlled conditions around physiological pH.

Common uses include preparing working-strength buffers by dilution, adjusting and stabilizing the pH of nucleic acid and protein solutions, equilibrating chromatography columns, performing enzyme reactions optimized near pH 7.5, and supporting electrophoresis and blotting protocols.

  • 1M Tris-HCl
  • Polymerase Chain Reaction (PCR): Used in PCR reactions to maintain optimal pH conditions for enzyme activity.

  • Gel Electrophoresis: Serves as a running buffer component in SDS-PAGE and native PAGE, providing consistent pH for protein separation.

  • Protein Purification: Utilized in anion exchange chromatography to maintain a stable pH environment during protein elution.

  • Enzyme Assays: Provides a consistent pH for enzymatic reactions, ensuring accurate kinetic measurements.

  • pH: 7.50 ± 0.04
Storage Temperature
Room Temperature
Shelf Life
1095 days
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