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T1062

10mM Tris-HCl, pH 8.5. 250mL, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

250mL

Item Count

1 Bottle(s)

Price per Item

$47.00

Fill Volume

250 mL

T1062

10mM Tris-HCl, pH 8.5. 250mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

10 mM Tris-HCl, pH 8.5 is a buffering solution used to maintain a mildly alkaline environment for biochemical and molecular biology procedures. It provides stable pH conditions suitable for handling nucleic acids and proteins in routine laboratory workflows.

Typical applications include elution and resuspension of DNA and RNA, preparation and dilution of samples for spectrophotometric measurements, adjustment of reaction conditions in enzyme-based protocols where Tris buffers are specified, and column equilibration or wash steps in compatible chromatography methods.

  • 10mM Tris-HCl
  • Nucleic Acid Elution and Resuspension: Elutes plasmid DNA from silica columns (EB) and resuspends DNA, RNA, and oligos at pH 8.5 for stability.

  • PCR/qPCR and RT Workflows: Used to dilute primers, probes, and templates; provides a defined pH component in reaction mixes.

  • NGS Library Preparation: Dilutes/adapts oligos and elutes amplicons and libraries in a low-salt, nuclease-compatible buffer.

  • Enzyme Assays and Dilutions: Maintains pH 8.5 for alkaline-optimum enzymes and prepares enzyme/substrate working stocks.

  • Protein Purification and Buffer Exchange: Serves as a low-ionic-strength buffer for column equilibration, dialysis, and desalting.

  • Sample Storage: Provides a gentle, low-salt medium for short- and mid-term storage of nucleic acids and proteins.

  • Plasmid Workflows: Resuspends and stores plasmid DNA from Escherichia coli preparations at pH 8.5.

  • pH: 8.50 ± 0.04
Storage Temperature
Room Temperature
Shelf Life
1095 days
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