Gel Electrophoresis (DNA/RNA): Used as an alkaline running buffer for agarose or polyacrylamide gels to improve resolution of nucleic acids at high pH.

Protein Electrophoresis: Serves as a running buffer in native or SDS-PAGE systems optimized for basic proteins, maintaining stable pH during separation.

Capillary Electrophoresis: Employed as a background electrolyte for peptide, amino acid, and small-molecule separations at alkaline pH.

Protein Purification: Used in anion exchange chromatography to maintain alkaline pH during binding and elution of proteins.

Enzyme Assays: Provides stable pH ~9.35 for enzymes requiring alkaline conditions (e.g., alkaline phosphatase, certain proteases).

Protein Crystallization: Functions as a buffering component in crystallization screens spanning pH 8–9.5.

Nucleic Acid Workflows: Suitable for RNA/DNA handling and sample preparation where DNase/RNase-tested, sterile buffer is required.

General Alkaline Buffering: 0.25M TAPS, Free Acid used to maintain consistent pH in biochemical and biophysical experiments around pH 9–9.5.