DNA Storage and Resuspension: Maintains stability of plasmid, genomic, and PCR-amplified DNA at pH 8.0 using 10 mM Tris-HCl, 1.0 mM EDTA.

RNA Storage (Short-Term): Provides nuclease inhibition via EDTA chelation during handling of RNA aliquots.

PCR/qPCR Template Dilution: Preserves nucleic acid integrity for sensitive amplification while minimizing nuclease activity.

NGS Library Handling: Used for resuspension, storage, and dilution of DNA libraries and adapters to reduce degradation.

Elution from Purification Media: Serves as an elution buffer for DNA recovery from silica spin columns and magnetic beads.

Plasmid Prep Resuspension: Resuspends purified plasmid DNA from Escherichia coli for downstream cloning, sequencing, or transfection.

Restriction/Enzymatic Reaction Quenching: Chelates Mg2+ to stop DNase I, exonuclease, or restriction enzyme reactions.

Transfection/Microinjection DNA Prep: Buffers DNA used for cell transfection or embryo microinjection to maintain integrity.

Spectrophotometric and Fluorometric Standards: Used as a blank or diluent for A260/280 and dye-based nucleic acid quantification.

Cloning and Gibson/Assembly Workflows: Stabilizes inserts, vectors, and oligos during setup and storage.