We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

T0224

TE Buffer (10mM Tris, 1mM EDTA), pH 8.0. 1mL, 50-Pack, Sterile. DNase/RNase/PCR

Availability

Ships in 1 - 2 Days

Container Size

2mL

Item Count

50 Tube(s)

Price per Item

$165.00

Fill Volume

1 mL

T0224

TE Buffer (10mM Tris, 1mM EDTA), pH 8.0. 1mL, 50-Pack, Sterile. DNase/RNase/PCR

Quantity

scroll

Description
Formulation
Common Applications
Quality Specifications
Storage

TE Buffer, pH 8.0, is a buffering solution used for the storage, dilution, and handling of DNA and RNA. Tris-HCl maintains a stable pH environment for nucleic acids, and EDTA chelates divalent cations such as Mg2+ to limit nuclease activity. Applicable to DNase/RNase-sensitive workflows and PCR setup and cleanup.

  • 10mM Tris-HCl
  • 1mM Ethylenediaminetetraacetic acid (EDTA)
  • Nucleic Acid Storage: Long-term storage of DNA and RNA at pH 8.0 using 10 mM Tris-HCl, 1 mM EDTA to maintain stability and inhibit nuclease activity.

  • DNA Resuspension: Rehydration of lyophilized or ethanol-precipitated DNA (genomic, plasmid, PCR products) for downstream applications.

  • PCR/qPCR Template Dilution: Stabilizes DNA templates for amplification while minimizing nuclease degradation (avoid excess carryover into Mg2+-dependent reactions).

  • Sequencing Sample Prep: Dilution and storage of Sanger and NGS libraries/amplicons to preserve integrity prior to loading.

  • Column/Gel Elution: Elution buffer for recovering DNA from silica columns or gel extraction workflows, improving DNA stability versus water.

  • Plasmid DNA Handling: Resuspension and storage of plasmids isolated from Escherichia coli for cloning, transfection, or archiving.

  • Oligonucleotide and cDNA Storage: Stabilizes ssDNA, dsDNA, and cDNA stocks and working dilutions under nuclease-free conditions.

  • Probe Preparation: Storage and dilution of labeled probes for Southern/Northern blotting and in situ hybridization.

  • Restriction/Ligation Sample Buffering: Storage and transport of DNA substrates prior to Mg2+-dependent restriction digests or ligations.

  • Spectrophotometric Quantification: Diluent for accurate A260/A280 measurements and standard curve preparations for DNA/RNA assays.

  • pH: 8.00 ± 0.04
  • Conductivity: 700-900µS/cm
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
1460 days
Plasmid Workflow

This product is part of our Plasmid Workflow

To expedite your plasmid production, we offer a wide variety of high-quality off-the-shelf and custom solutions, from selective culture media to buffers and reagents that can be used throughout the pDNA growth and purification process.

  • Selection and Culturing
  • Purification and Recovery

Shows related citations for this product
Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button