Selection of Kanamycin-Resistant Transformants: Isolating colonies harboring kanamycin-resistant plasmids (e.g., Escherichia coli, Bacillus subtilis, Pseudomonas putida).

Maintenance of Recombinant Strains: Routine passaging of engineered bacteria under 50 µg/mL kanamycin selection to preserve plasmids or genomic insertions.

Colony Screening for Cloning Workflows: Screening ligation/Gibson assemblies by growth on selective TSA to confirm acquisition of kan-marked constructs.

Transformation Efficiency and CFU Enumeration: Quantifying transformants by plating serial dilutions on kanamycin-selective agar.

Conjugation and Transduction Selection: Recovering transconjugants or transductants carrying kanamycin resistance markers.

Transposon Mutagenesis Selection: Selecting insertion mutants carrying a kanamycin resistance cassette.

Streaking for Single Colonies: Isolating clonal lines of kan-resistant strains (e.g., Escherichia coli, Salmonella enterica).

Mixed Culture Enrichment: Enriching target kan-resistant strains from mixed samples while suppressing non-resistant contaminants.

Plasmid Stability Assays: Assessing retention of kan-marked plasmids across passages under selective pressure.

Stock Preservation and Transport: Preparing and archiving colonies of kan-resistant isolates (e.g., Acinetobacter baylyi) for short-term storage.