Selective Isolation of Chloramphenicol-Resistant Bacteria: Recovery and maintenance of cat-marked strains (e.g., Escherichia coli, Staphylococcus aureus, Bacillus subtilis) from mixed cultures.

Transformant/Transconjugant Screening: Selection of colonies carrying chloramphenicol-resistant plasmids or genome insertions following cloning or conjugation.

Colony Isolation and CFU Enumeration: Plating for single colonies and quantification of chloramphenicol-resistant cells from cultures or libraries.

Background Suppression in Mixed Samples: Counter-selection against chloramphenicol-susceptible flora in environmental, food, or process samples.

Strain Maintenance and Short-Term Storage: Routine streaking and passage of cat-marked production and research strains on stable solid medium.

Quality Control and Stability Testing: Monitoring retention of chloramphenicol resistance markers in engineered strains across passages.

Antibiotic Resistance Verification: Confirmation of chloramphenicol resistance phenotype for clinical, industrial, or research isolates (e.g., Pseudomonas aeruginosa with cat marker).

Enrichment of Fungi and Yeasts: Recovery of molds and yeasts from samples by inhibiting bacterial overgrowth with 15 µg/mL chloramphenicol.

Defined Consortia and Co-Culture Studies: Maintenance of specific cat-marked members within microbial communities on a general-purpose nutrient base.