Selective Isolation of Kanamycin-Resistant Bacteria: Tryptic Soy Agar (1.5% Tryptone, 0.5% Soytone, 0.5% Sodium Chloride, 1.5% Agar) with 25µg/mL kanamycin for isolating resistant strains such as Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa.

Plasmid Maintenance and Cloning: Maintains plasmids carrying kanR markers in hosts like E. coli during routine subculture and cloning workflows.

Transformation and Electroporation Screening: Post-transformation selection of kanamycin-resistant colonies from competent E. coli or other host strains.

Conjugation and Transconjugant Selection: Recovery and selection of kanR transconjugants in mating experiments (e.g., Agrobacterium tumefaciens to E. coli or other bacteria).

CFU Enumeration Under Selection: Quantification of colony-forming units from serial dilutions to measure frequencies of kanamycin-resistant cells.

Colony Purification and Streaking: Restreaking recombinant strains to ensure clonal purity while maintaining selection pressure.

Transposon Mutant Library Maintenance: Selection and propagation of Tn5/Tn-kanR mutant libraries in organisms such as E. coli or Salmonella enterica.

Verification of Genetic Constructs: Screening for correct insertion/maintenance of kanR-tagged cassettes, reporters, or expression vectors.

Strain Stability and Quality Control: Periodic plating to confirm retention of kanR plasmids or chromosomal markers in lab strains.

Environmental or Clinical Isolate Selection: Enrichment and isolation of kanamycin-resistant bacteria from mixed samples when selective pressure is required.