Routine Cultivation of Nonfastidious Bacteria: General-purpose growth of aerobes and facultative anaerobes such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa.

Isolation of Pure Cultures: Streak plating for colony isolation and morphology assessment.

Colony Enumeration (CFU): Spread plating and serial dilution plating for quantifying viable bacteria.

Environmental and Bioburden Monitoring: Recovery of aerobic heterotrophs from surfaces, air, water, and process samples.

Quality Control of Strains: Routine verification of colony morphology, pigmentation, and contamination checks.

Starter Cultures for Downstream Testing: Preparing colonies for biochemical assays, Gram staining, and molecular workflows.

Maintenance and Short-Term Storage: Holding working stocks of laboratory strains on plates.

Phenotypic Screening with Supplements: Selection or indicator-based screening when TSA is supplemented with antibiotics or dyes.

Replica Plating and Colony Picking: High-throughput transfer and picking for cloning and library maintenance.

Recovery of Stressed Cells: Cultivation of mildly stressed or sublethally injured bacteria after sample processing.