Buffer Preparation (pH Control): Stock solution for preparing citrate buffers at pH 6.0 to maintain stable conditions in biochemical and molecular biology workflows.

Enzyme Assays: Provides a consistent pH 6.0 environment (citrate buffer) for acid-active enzymes and for chelating divalent cations that affect enzyme activity.

Antigen Retrieval (IHC): Used as citrate buffer, pH 6.0, for heat-induced epitope retrieval in immunohistochemistry on formalin-fixed, paraffin-embedded tissues.

Nuclease Inhibition: Chelates Mg2+ to help reduce RNase/DNase activity and stabilize nucleic acids during extraction and storage.

Microbiological Differentiation Tests: Component and carbon source in Simmons citrate agar for identifying members of Enterobacteriaceae (e.g., Escherichia coli, Klebsiella pneumoniae).

Anticoagulation/Cell Processing: Utilized to chelate Ca2+ in blood and platelet handling workflows to prevent coagulation (prepare working dilutions from 1M Sodium Citrate).

Protein and Metalloprotease Studies: Applied as a mild chelating buffer to modulate activity of metal-dependent proteins and proteases in assays.

Nanoparticle Synthesis: Source of citrate ions for the reduction and stabilization of gold nanoparticles (Turkevich method) using sterile 1M Sodium Citrate stock.