DNA/RNA Precipitation and Cleanup: 200mM Sodium Acetate, pH 5.5 is commonly added prior to ethanol or isopropanol to precipitate and wash nucleic acids.

Enzyme Assays at Acidic pH: Provides a stable pH 5.5 environment with 200mM Sodium Acetate for acid-optimal enzymes and activity screens.

Cation Exchange Chromatography: Used as equilibration and elution buffer (pH 5.5) for proteins and capsids on SP/CM resins; 200mM Sodium Acetate maintains ionic strength.

Viral Vector Purification/Formulation: 200mM Sodium Acetate, pH 5.5 is frequently used for AAV purification steps and low-pH formulation buffers.

Buffer Exchange and Dialysis: Maintains pH 5.5 during desalting/dialysis to condition samples for downstream processing.

Protein Stabilization/Storage: Supports stability of acid-stable proteins/peptides at pH 5.5; 200mM Sodium Acetate provides consistent buffering capacity.

Ion-Exchange HPLC Methods: Serves as a pH 5.5 mobile/buffer component where non-volatile salts are acceptable; 200mM Sodium Acetate controls ionic strength.

Endotoxin-Sensitive Workflows: Sterile, endotoxin-tested 200mM Sodium Acetate, pH 5.5 is suited for LAL-compatible buffers and bioprocess steps requiring low endotoxin.