Fungal Isolation and Enumeration: Recovers yeasts and molds from clinical and environmental specimens using 1.0% tryptone, 4.0% dextrose, 1.5% agar; acidic conditions help suppress bacteria like Escherichia coli and Staphylococcus aureus.

Environmental Monitoring/Air Sampling: 150 mm plates used as settle plates or in active air samplers for airborne yeast and mold surveillance in cleanrooms and production areas.

Dermatophyte Recovery: Isolation of skin, hair, and nail pathogens (e.g., dermatophytes) from clinical specimens for diagnosis.

Food and Beverage Quality Control: Detection and enumeration of yeasts and molds in foods, beverages, and dairy products.

Colony Morphology and Sporulation Studies: Assessment of fungal colony characteristics, pigmentation, and sporulation; maintenance of fungal stock cultures.

Bioburden and Microbial Limits Testing: Quantification of fungal contamination in pharmaceuticals, cosmetics, and personal care products.

Pre-Susceptibility Culture: Growth and recovery of fungal isolates prior to antifungal susceptibility testing workflows.

Selectivity in Mixed Cultures: Preferential recovery of fungi from bacteria-rich samples; can be supplemented to further inhibit Pseudomonas aeruginosa and other bacteria.

Inoculum Preparation for Identification: Cultivation of pure fungal colonies for downstream ID methods (microscopy, biochemical panels, MALDI-TOF).

Environmental and Clinical Mycology Teaching: Routine mycology exercises demonstrating yeast and mold growth under acidic, high-dextrose conditions.