Nucleic Acid Precipitation: Facilitates efficient ethanol/isopropanol precipitation of DNA and RNA at acidic pH for high-yield recovery.

Phenol–Chloroform Extractions: Provides controlled pH and ionic strength to improve phase separation and nucleic acid recovery.

TAE/Acetate Buffer Preparation: Serves as the acetate component to formulate acetate-based running and wash buffers for nucleic acid workflows.

Ion-Exchange Chromatography: Used as an acetate buffer at pH 5.5 for cation-exchange binding and elution of proteins and peptides.

Enzyme Assays: Provides a defined pH 5.5 acetate environment for enzymes with acidic optima, ensuring reproducible kinetics.

Protein Formulation and Storage: Acts as a stabilizing acetate buffer for sensitive proteins where chloride or phosphate are undesirable.

Oligonucleotide Purification: Used as a low-UV mobile phase/buffer component for HPLC-based oligo desalting and fractionation.

Endotoxin-Sensitive Workflows: Sterile, endotoxin-tested buffer suitable for preparing reagents used in mammalian cell culture and downstream assays.

Plasmid DNA Cleanup: Employed in DNA precipitation and buffer exchange steps during plasmid prep from Escherichia coli cultures.

Gel Extraction and Cleanup: Helps adjust pH and ionic strength to promote DNA binding/release in silica-based purification methods.