Protein A/G Affinity Chromatography Elution: Uses 0.1M Sodium Citrate, pH 3.0 to efficiently elute IgG from Protein A/G resins with rapid post-elution neutralization.

Viral Inactivation (Low-pH Hold): Provides a controlled pH 3.0 environment for enveloped virus inactivation during downstream bioprocessing.

AAV and Lentiviral Vector Processing: Employed for low-pH capsid elution and pH-shift steps, followed by immediate neutralization to preserve vector integrity.

HPLC/UPLC Mobile Phase Buffering: Serves as a pH 3.0 buffer for peptide/protein separations and reversed-phase workflows to improve peak shape and reproducibility.

Acid Dissociation in Immunoassays: Used to dissociate antibody–antigen complexes (e.g., ELISA, bridging assays) prior to detection or re-binding.

Endotoxin Handling and Recovery Studies: Endotoxin-tested, sterile matrix for LAL inhibition/enhancement controls and endotoxin spike/dilution preparations.

Enzyme and Nuclease Inactivation: Low pH and citrate chelation help suppress proteases and nucleases during sample preparation.

Ion-Exchange Chromatography pH Control: Maintains pH 3.0 conditions for cation-exchange equilibration and elution strategies.

Host-Cell Protein Clarification: Facilitates precipitation/clarification of host-cell proteins from Escherichia coli or CHO lysates under acidic conditions.

Antibody/Fc Interaction Stripping: Low-pH treatment to strip surface-bound antibodies or Fc-mediated interactions from cells or tissue prior to re-staining.