Polymerase Chain Reaction (PCR): Used in PCR reactions to maintain optimal pH conditions for enzyme activity.

Gel Electrophoresis: Serves as a running buffer in SDS-PAGE and native PAGE, providing consistent pH for protein separation.

Protein Purification: Utilized in anion exchange chromatography to maintain a stable pH environment during protein elution.

Enzyme Assays: Provides a consistent pH for enzymatic reactions, ensuring accurate kinetic measurements.

Preparation of PBS and Working Buffers: Used as a 1M Potassium Phosphate Buffer stock to formulate PBS and other dilution/storage buffers at physiological pH.

Cell and Bacterial Culture: Buffers growth media for robust culture of Escherichia coli and other microbes at pH 7.2.

Immunoassays (ELISA/Western Blot): Stabilizes pH in wash, blocking, and antibody incubation buffers for reproducible signal.

Nucleic Acid Workflows: Maintains pH in DNA/RNA extraction, cleanup, and storage buffers to preserve integrity.

Spectrophotometric/Colorimetric Assays: Provides a stable pH matrix for assays such as Bradford, BCA, and enzyme activity measurements.

General Sample Handling: Used for sample dilution and storage to minimize pH-induced degradation of biomolecules.