Nucleic Acid Precipitation: Used with ethanol or isopropanol to efficiently precipitate and concentrate DNA and RNA from aqueous solutions.

Plasmid DNA Cleanup: Facilitates precipitation and cleanup of plasmid DNA following restriction digests, ligations, and enzymatic reactions.

Phenol–Chloroform Extraction: Provides acidic buffering during phenol–chloroform workflows to enrich and recover RNA.

Cation Exchange Chromatography: Serves as a low-pH acetate buffer for protein purification and elution on cation exchange resins.

Protein Crystallization: Functions as a buffering component near pH 5.2 in crystallization screens for optimizing protein crystal growth.

Small RNA Recovery: Enhances glycogen-assisted precipitation to improve yield of small RNAs and low-abundance nucleic acids.

DNA Concentration and Desalting: Enables precipitation-based concentration and removal of salts from DNA samples prior to downstream analyses.

HPLC Buffering: Acts as an aqueous-phase buffer for chromatographic separation of biomolecules requiring acetate buffering at pH 5.2.