SDS-PAGE: Denatures and imparts uniform negative charge to proteins for size-based separation.

Western Blotting: Maintains protein denaturation in sample/loading buffers and aids in membrane stripping.

Cell Lysis: Disrupts cell membranes for protein and nucleic acid extraction from Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa.

Membrane Protein Solubilization: Extracts and solubilizes hydrophobic and membrane-associated proteins.

Nucleic Acid Purification: Facilitates protein removal during DNA/RNA isolation (e.g., with Proteinase K or phenol–chloroform workflows).

Chromatin and ChIP Lysis Buffers: Provides stringent lysis for chromatin shearing and immunoprecipitation workflows.

Viral and Pathogen Inactivation: Disrupts lipid envelopes and denatures proteins for safe sample handling.

Protein Quantitation Prep: Ensures complete protein denaturation for SDS-compatible assays and standard preparation.

Sample Decontamination: Helps decontaminate labware and inactivate residual proteins and nucleases in preparations.

General Detergent in Harsh Lysis Buffers: Enhances stringency in buffers requiring strong anionic surfactant action.