Protein Purification: Utilized in anion exchange chromatography to maintain stable pH during protein binding and elution with 100mM Sodium Phosphate Buffer.

Protein Sample Preparation and Storage: Provides a consistent pH 8.5 environment for diluting, stabilizing, and storing proteins with 100mM Sodium Phosphate Buffer.

Enzyme Assays: Maintains alkaline conditions suitable for enzymes with pH optima near 8–9, such as alkaline phosphatase, using 100mM Sodium Phosphate Buffer.

Antibody Conjugation and Labeling: Supports NHS-ester and other amine-reactive chemistries at pH 8.5 with 100mM Sodium Phosphate Buffer.

ELISA and Immunoassays: Used as a coating/dilution buffer component and for preparing PBST-style wash buffers with 100mM Sodium Phosphate Buffer.

Buffer Exchange and Dialysis: Employed as the target buffer to equilibrate protein samples to pH 8.5 using 100mM Sodium Phosphate Buffer.

pH Control in Biochemical Reactions: Provides robust buffering capacity around pH 8.5 to enhance reaction reproducibility with 100mM Sodium Phosphate Buffer.

Cell and Bacterial Sample Washing: Sterile buffer for washing or resuspending cells, including E. coli and Bacillus subtilis, using 100mM Sodium Phosphate Buffer.

Chromatography System Equilibration: Used to equilibrate columns, tubing, and detectors at defined pH prior to runs with 100mM Sodium Phosphate Buffer.

Native Protein Electrophoresis: Serves as a buffering system for certain native PAGE formats requiring phosphate at pH 8.5 using 100mM Sodium Phosphate Buffer.