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S0050

150mM Sodium Phosphate Buffer, pH 8.5, 1000mL, Sterile, DNase/RNase Tested

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$94.00

Fill Volume

1000 mL

S0050

150mM Sodium Phosphate Buffer, pH 8.5, 1000mL, Sterile, DNase/RNase Tested

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

150 mM Sodium Phosphate Buffer, pH 8.5, DNase/RNase Tested is a ready-to-use buffering solution for pH control in biochemical and molecular biology workflows, including sample preparation, dilution, and chromatography steps requiring an alkaline phosphate buffer.

Each lot is tested for the absence of detectable DNase and RNase activity. Store at 2–8 °C. Mix gently before use. For laboratory use.

  • 150mM Sodium Phosphate
  • Enzyme Assays: Provides stable pH 8.5 in 150mM Sodium Phosphate for enzymatic reactions not inhibited by phosphate.

  • Protein Purification: Used for anion exchange chromatography equilibration, wash, and elution at pH 8.5.

  • Bioconjugation and Labeling (NHS-ester): Amine-free 150mM Sodium Phosphate, pH 8.5 supports efficient protein and antibody coupling.

  • HPLC/UPLC Mobile Phases: Serves as a buffered mobile phase component at 150mM, pH 8.5 for reproducible separations.

  • Sample Preparation and Dilution: Sterile, DNase/RNase-tested diluent for nucleic acid and protein handling.

  • Dialysis and Buffer Exchange: Maintains pH 8.5 during desalting, diafiltration, and buffer exchange of proteins.

  • Immunoassays (ELISA) and Blot Washes: Base buffer to prepare PBST/PBS-style wash buffers by adding NaCl and Tween.

  • Protein Storage/Stabilization: Provides an alkaline environment (pH 8.5) to stabilize proteins requiring basic conditions.

  • Chromatography Sample Conditioning: Adjusts and maintains sample pH at 8.5 before anion exchange or affinity workflows.

  • pH: 8.50 ± 0.04
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
1095 days
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