Heterotrophic Plate Count (HPC) in potable and bottled water: Supports enumeration of heterotrophic bacteria from low-nutrient waters under extended, low-temperature incubation.

Recovery of stressed/injured waterborne bacteria: Facilitates resuscitation of chlorine- or UV-stressed cells from treated water distribution systems.

Cultivation of oligotrophic/environmental bacteria: Enriches slow-growing heterotrophs from surface water, groundwater, and biofilms.

Most Probable Number (MPN) assays: Used as a low-nutrient medium for MPN-based enumeration of drinking and environmental water microbiota.

Pre-enrichment before plating: Resuscitates and acclimates organisms prior to plating on R2A agar or other low-nutrient media.

Process monitoring in water treatment and bottling: Tracks microbial loads to assess treatment efficacy and distribution system hygiene.

Biofilm studies: Cultures low-nutrient biofilm communities from pipes, RO systems, and medical water lines.

Membrane filtration workflows: Serves for filter rinsing/resuscitation steps prior to colony development on low-nutrient agar.

Serial dilutions and sample blending (2X use): Employed at double strength for 1:1 mixing with samples to achieve 1X final concentration.

Long-term incubation studies: Supports extended incubation (e.g., 20–28°C, several days) to recover slow-growing waterborne heterotrophs.