Heterotrophic Plate Count (HPC) in Water Testing: Low-nutrient medium for enumerating heterotrophic bacteria from potable, purified, and distribution system waters.

Recovery of Stressed/Slow-Growing Bacteria: Promotes recovery of chlorine-, UV-, and nutrient-stressed organisms from treated water systems.

Oligotrophic Bacteria Cultivation: Supports growth of slow-growing oligotrophs such as Sphingomonas, Pseudomonas, Acinetobacter, and Caulobacter.

Membrane Filtration and MPN Assays: Used as a low-nutrient recovery broth for membrane filter rinses and most probable number determinations.

Environmental Monitoring of Water Systems: Suited for monitoring distribution lines, biofilms, building plumbing, and cooling water.

Beverage and Bottled Water QC: Enumeration and recovery of background microbiota in bottled and low-nutrient beverages.

Pharma/Medical Water Testing: Assessment of purified water, WFI, and dialysis water for low-level microbial contamination.

Sample Pre-Enrichment and Dilution: Used as a non-inhibitory diluent and enrichment broth prior to R2A plating.

Biofilm Studies: Cultivation of low-nutrient biofilm communities from environmental and engineered water systems.