Enzyme Assays: Provides a stable pH 6.0 environment for enzymes with acidic optima, enabling reproducible kinetic measurements.

Protein Purification: Used as equilibration, wash, and elution buffer for ion-exchange chromatography near pH 6.0 to preserve protein stability.

Protein Storage and Dialysis: Maintains protein conformation and activity during storage, buffer exchange, and desalting at mildly acidic pH.

HPLC Mobile Phase: Serves as a phosphate buffer component for reproducible retention and peak shape in reversed-phase and ion-exchange HPLC at pH 6.0.

Sample Preparation and Dilution: Acts as a 1 M stock to adjust pH and ionic strength when preparing working buffers at or near pH 6.0.

Cell and Tissue Homogenization: Buffers extracts during lysis and clarification to protect pH-sensitive biomolecules.

Microbiology Media Buffering: Stabilizes culture media at mildly acidic pH for organisms and workflows that require pH 6.0; sterile format supports aseptic applications.