Ion-Exchange Chromatography: Used to prepare working phosphate buffers (e.g., 10–100 mM, pH 6.0) that maintain stable pH during anion or cation exchange separations.

Size-Exclusion Chromatography (SEC): Serves as a stock to make isocratic mobile phases at pH 6.0 for protein and peptide SEC workflows.

Protein Purification and Storage: Diluted from 1M to typical working strengths to stabilize proteins during purification, dialysis, and storage at pH 6.0.

Enzyme Assays: Provides a defined pH 6.0 environment for enzymes with acidic optima, supporting reproducible kinetics.

Microbial Culture and Sample Preparation: Used for pH control and cell resuspension/dilution in workflows involving Escherichia coli, Bacillus subtilis, and Staphylococcus aureus.

Cell Lysis and Homogenization: Forms the buffering base for lysis buffers at pH 6.0 to preserve target biomolecules during extraction.

HPLC/UPLC Aqueous Phase Preparation: Stock for preparing phosphate-based aqueous phases (commonly 10–50 mM) at pH 6.0 for biomolecule separations.

General pH Control and Titration: Employed as a 1M stock to quickly adjust and maintain solutions at pH 6.0 in analytical and preparative workflows.