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P0268

1X PBS Solution, pH 7.9. 1000mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$99.00

Fill Volume

1000 mL

P0268

1X PBS Solution, pH 7.9. 1000mL, Sterile.

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

1X PBS Solution, pH 7.9, is an isotonic phosphate-buffered saline composed of sodium chloride, potassium chloride, and phosphate salts. It provides a stable, slightly alkaline environment suitable for routine laboratory procedures.

Applications include washing cells and tissues, diluting samples and reagents, preparing blanks and standards, resuspending cell pellets, and rinsing membranes in blotting and immunoassays across molecular biology and microbiology workflows, including handling of Escherichia coli.

  • 135mM Sodium chloride
  • 2.7mM Potassium chloride
  • 4.3mM Disodium phosphate
  • 1.4mM Monopotassium phosphate
  • Cell Culture Washing: Rinses adherent or suspension cells to remove serum and enzymes without disrupting osmotic balance.

  • Sample Dilution: Dilutes proteins, nucleic acids, and reagents while maintaining isotonicity and near-neutral pH.

  • Immunoassays (ELISA): Used for plate washing and reagent dilution to reduce nonspecific binding and preserve antigen-antibody interactions.

  • Western Blotting: Serves as a wash buffer and antibody diluent to minimize background during blot detection.

  • Flow Cytometry: Suspends and washes cells to maintain viability and minimize cell aggregation during analysis.

  • Immunofluorescence/IHC: Washes tissues and cells between staining steps to preserve morphology and signal specificity.

  • Microscopy: Provides a clear, isotonic medium for mounting, washing, and imaging live or fixed cells and tissues.

  • Cell and Microbial Pellet Washing: Rinses cell pellets to remove media components prior to lysis, extraction, or transformation.

  • Nucleic Acid and Protein Handling: Resuspends and stores DNA, RNA, or proteins short-term at controlled pH and ionic strength.

  • Instrument and Surface Rinsing: Rinses tubing, cuvettes, and benchtop surfaces to remove residual reagents between runs.

  • pH: 7.9 ± 0.1
Storage Temperature
Room Temperature
Shelf Life
1095 days
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