Cell and Tissue Washing: Isotonic rinse for removing media, serum, or reagents while maintaining osmotic balance at mildly acidic pH 6.0.

Sample Dilution: Diluent for proteins, antibodies, and analytes where a slightly acidic environment is preferred.

ELISA and Plate Washing: Wash buffer base for microplates; pH 6.0 can help reduce nonspecific binding in certain assays.

Western Blot and Antibody Incubations: Buffer base for membrane washes and antibody dilutions when acidic conditions are required.

Flow Cytometry: Staining and wash buffer base (often with serum/azide added) maintaining ionic strength and pH control.

Immunofluorescence/IHC Washes: Tissue/slide washing between staining steps where pH 6.0 supports epitope stability.

Histology and Tissue Handling: Rinsing sections or tissues to preserve morphology and minimize osmotic stress.

Microbiology Cell Washing: Washing and resuspension of bacterial pellets (e.g., Escherichia coli, Staphylococcus aureus) without altering ionic strength.

Preparation of 1X PBS: Convenient 1:10 dilution to obtain working PBS (pH 6.0) with 135 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4.

General Laboratory Rinse/Transport Buffer: Maintaining physiological salt conditions for cells, tissues, and biomolecules during short transfers.