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O9710

Stain Extraction Buffer with Sarkosyl. 1000mL, DNase Tested, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$64.00

Fill Volume

1000 mL

O9710

Stain Extraction Buffer with Sarkosyl. 1000mL, DNase Tested, Sterile.

Quantity

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Description
Formulation
Common Applications
Storage

Stain Extraction Buffer with Sarkosyl, DNase Tested is a ready-to-use solution formulated for detergent-assisted disruption of cellular and sample matrices to facilitate recovery of bound or embedded stains from biological preparations. The composition provides buffering capacity, controlled ionic strength, chelation of divalent cations, and an anionic surfactant for solubilization of proteins and membrane components.

Each lot is tested for DNase activity to support workflows in which preservation of nucleic acids is required. The buffer is suitable for processing cell lysates, tissue homogenates, membrane fractions, and gel-based samples in laboratory protocols that require reproducible stain extraction under defined conditions.

  • 100mM Sodium Chloride
  • 50mM Tris-HCl
  • 50mM EDTA
  • 2% Sodium Lauroyl Sarcosine
  • Nucleic Acid Gel Destaining: Extracts intercalating dyes (e.g., ethidium bromide, SYBR dyes) from agarose gel slices to improve downstream DNA recovery.

  • DNA Purification Prep: Removes residual stains from DNA samples prior to ligation, cloning, or sequencing to reduce inhibition.

  • Protein Gel Destaining Aid: Assists in extracting Coomassie and other dyes from polyacrylamide gels for clearer background.

  • Cell Lysis for Nucleic Acid Prep: Detergent-assisted lysis and EDTA chelation support extraction of DNA from bacteria such as Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.

  • PFGE Plug Cleanup: Helps remove residual stains and cellular debris from agarose plugs prior to pulsed-field gel electrophoresis.

  • PCR/Inhibitor Removal: Reduces dye-related inhibitors in templates prior to PCR and qPCR for improved amplification.

  • Fluorescent Stain Recovery: Facilitates extraction and analysis or disposal of fluorescent nucleic acid dyes from gels or solutions.

  • Sample Clarification: Enhances clarity of nucleic acid preparations by solubilizing hydrophobic contaminants and stabilizing pH/ionic strength.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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