General Cultivation: Non-selective growth of non-fastidious bacteria (e.g., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis).

Inoculum Preparation: Generating starter cultures for plating, biochemical tests, and downstream workflows.

Enrichment and Recovery: Resuscitation of stressed or starved cells from food, water, and environmental samples (e.g., Salmonella enterica, Enterobacter cloacae).

Growth Promotion Testing: QC verification of media performance with standard organisms (e.g., E. coli, Staphylococcus aureus).

Sterility Testing: Non-selective medium for detecting microbial contamination in process and product samples.

Antimicrobial Studies: Baseline growth controls and pre-cultures for MIC/MBC and disinfectant efficacy testing.

Enumeration and Growth Curves: Turbidity/OD600 measurements for counting and kinetic studies.

Culture Maintenance: Short-term propagation and subculture of laboratory strains (e.g., Klebsiella pneumoniae, Enterococcus faecalis).

Seed Cultures for Fermentation: Preparing inocula for small-scale fermentation and recombinant workflows.

Environmental Monitoring: Recovery medium for surface/swab samples and bioburden assessments.

Phage Workflows: Growing bacterial hosts for bacteriophage propagation and assays.

Education and Training: Teaching labs for basic microbial cultivation and media preparation skills.