Routine Cultivation of Nonfastidious Bacteria: Supports growth of organisms such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa.

Preparation of Inocula: Used to generate starter cultures for downstream assays and microbiological workflows.

Enumeration and Serial Dilutions: Serves as a non-selective medium for serial dilution and viable plate count procedures.

Growth Promotion and QC Testing: Employed in quality control to verify microbial recovery and medium performance.

Pre-Enrichment of Samples: Facilitates recovery of stressed or low-level organisms from food, water, or environmental samples prior to selective plating.

Environmental Monitoring Recoveries: Used to resuspend and recover organisms from surface swabs and contact plates.

Antimicrobial Susceptibility Inoculum Prep: Provides a consistent matrix for preparing standardized inocula (e.g., 0.5 McFarland) for AST workflows.

Growth Curves and Physiology Studies: Enables measurement of microbial growth kinetics and basic physiological assays.

Short-Term Culture Maintenance: Supports temporary holding and subculture of laboratory strains.

Rehydration of Lyophilized Cultures: Used to revive and propagate preserved bacterial strains such as Salmonella enterica and Enterococcus faecalis.