Bacterial Plasmid Selection: Selects transformants carrying neo/kan resistance genes in E. coli, Bacillus subtilis, and Pseudomonas using 50mg/mL Neomycin Sulfate Solution in agar or broth.

Plasmid Maintenance in Culture: Maintains selection pressure to preserve plasmids with neomycin resistance during growth of E. coli and other bacteria.

Plant Tissue Culture Selection: Selects transgenic plant tissues expressing the neo gene in callus and regeneration media.

Transposon Mutagenesis Selection: Enables selection of insertion mutants carrying neomycin resistance cassettes in bacterial libraries.

Antibiotic Susceptibility Testing: Used as a reference aminoglycoside in MIC assays and quality control testing.

Contamination Control in Cultures: Suppresses sensitive bacterial contaminants in microbial and algal cultures when combined with aseptic techniques.