General Cultivation of Nonfastidious Bacteria: Supports routine growth and propagation of organisms such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa.

Overnight and Starter Cultures: Used to generate inocula for downstream workflows (e.g., plating, transformations) from strains like Escherichia coli.

Inoculum Preparation for Assays: Provides a consistent, isotonic medium to standardize cell density before biochemical tests or fermentation studies.

Enrichment from Mixed or Low-Biomass Samples: Promotes recovery and amplification of bacteria from environmental, food, or water samples prior to selective plating (e.g., Salmonella enterica screening).

Maintenance and Subculturing of Stocks: Suitable for short-term culture maintenance, revival, and routine passage of laboratory strains.

Microbial Load and Enumeration Workflows: Employed for pre-enrichment and serial dilution–based plate counts in quality control testing.

Sterility and Process Control Testing: Serves as a nonselective medium to detect microbial contamination in media fills, rinse fluids, and manufacturing environments.

Recovery of Stressed or Injured Cells: Facilitates resuscitation of bacteria following cold storage, dehydration, or disinfectant exposure.

Teaching and Training Applications: Common in academic labs for demonstrating basic bacteriology techniques and growth kinetics.

Diluent and Suspension Medium: Used to prepare uniform bacterial suspensions for standardizing inocula (e.g., McFarland-based setups).