C. elegans RNAi by Feeding: Supports RNA interference screens using E. coli HT115(DE3) + L4440 plasmids; 50µg/mL carbenicillin maintains AmpR plasmids and 1mM IPTG induces dsRNA expression.

Gene Knockdown Phenotype Screening: Enables systematic assessment of developmental, behavioral, and morphological phenotypes in C. elegans under induced RNAi conditions.

Genetic Interaction and Modifier Screens: Facilitates combinatorial RNAi to probe epistasis and pathway interactions in worms.

Tissue- or Stage-Specific RNAi Studies: Used with sensitized worm strains to examine temporal and tissue-restricted gene function on induced RNAi lawns.

Plasmid Maintenance in Feeding Strains: Carbenicillin selection stabilizes RNAi plasmids in E. coli HT115(DE3) during culture and plating.

Inducible Expression on Lawns: IPTG drives T7-based transcription in HT115(DE3), enhancing dsRNA yield for robust knockdown.

Routine C. elegans Growth and Maintenance: NGM formulation (0.25% Tryptone, 0.30% NaCl, 25mM phosphate, 1mM MgSO4/CaCl2, 2.00% agar, 5µg/mL cholesterol) supports healthy worm cultivation with defined bacterial lawns.

Quality-Controlled Feeding Libraries: Suitable for arrayed or pooled RNAi feeding collections of E. coli clones under antibiotic selection and IPTG induction.

High-Throughput Phenotyping: Compatible with multi-plate screening of gene function using standardized induced lawns.

Comparative Strain Studies: Allows side-by-side testing of E. coli HT115(DE3) versus non-inducible food strains (e.g., E. coli OP50) for control experiments.