2M MOPS Buffer, pH 7.4, is a concentrated Good’s buffer stock for preparing near-neutral aqueous buffering systems. MOPS (3-(N-morpholino)propanesulfonic acid) has a pKa of approximately 7.2 at 25 °C, providing buffering capacity in the pH 6.5–7.9 range. The 2 M concentration allows preparation of working solutions at commonly used ranges (e.g., 10–100 mM) by volumetric dilution with water or salt solutions.

This buffer is used in biochemical assays, protein and nucleic acid workflows, electrophoresis systems employing MOPS chemistry, and cell culture media where a zwitterionic, low-metal-binding buffer at physiological pH is needed. The pH is adjusted to 7.4 at 25 °C. Prepare working buffers by diluting the 2 M stock to the desired molarity; for example, a 50 mM MOPS buffer is obtained by a 1:40 dilution.